MABT1487

Anti-E-Cadherin/CD324 Antibody, clone 19A11

• Synonym(s): CAM 120/80, CD324, Cadherin-1, Epithelial cadherin, Uvomorulin
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 19A11, monoclonal
• mol wt: calculated mol wt 97 kDa
• purified by: using protein G
• species reactivity: human, canine
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal extracellular domain
• Protein ID accession no.: NP_004351
• UniProt accession no.: P12830
• storage temp.: -10 to -25°C
• Gene Information: human ... CDH1(999)

Description

Specificity

Clone 19A11 is a mouse monoclonal antibody that detects E-Cadherin/CD324. It targets an epitope within the extracellular domain.

Immunogen

Recombinant protein corresponding to the extracellular domain of human E-Cadherin.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected E-Cadherin/CD324 in MCF-7 cells.

Tested Applications

Agonist Function:: A representative lot stimulated adhesion of cells to pure E-Cadherin substrate (Petrova, Y., et al. (2012). Mol Biol Cell.;23(11):2092-108).

Western Blotting Analysis: A representative lot detected E-Cadherin/CD324) in Western Blotting applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108; Xie, B., et al. (2022). Proc Natl Acad Sci USA. 119(32):e2204473119).

Immunocytochemistry Analysis: A representative lot detected E-Cadherin/CD324 in Immunocytochemistry applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108).

ELISA Analysis: A representative lot detected E-Cadherin/CD324 in ELISA applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Cadherin-1 (UniProt: P12830; also known as CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324) is encoded by the CDH1 (also known as CDHE, UVO) gene (Gene ID: 999) in human. Cadherins are calcium-dependent cell adhesion molecules that participate in cell-cell adhesion during embryogenesis, development, organogenesis, and differentiation. E-cadherin is a single-pass type I membrane glycoprotein with tumor suppressive properties. It is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 23-154) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 155-709), a transmembrane domain (aa 710-730), and a cytoplasmic domain (aa 731-882). N-glycosylation at Asn-637 is shown to be essential for its expression, folding, and trafficking. E-cadherin is known to contain five cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Post-translationally it can be cleaved into three chains: E-Cad/CTF1 (aa 701-882); E-Cad/CTF2 (aa 732-882); and E-Cad/CTF3 (aa 751-882). During apoptosis or with calcium influx, it is cleaved by a membrane-bound metalloproteinase (ADAM10; at residues 700-701), which causes disruption of cell-cell adhesion and the subsequent release of β-catenin into the cytoplasm. The residual membrane-tethered cleavage product is then rapidly degraded via an intracellular proteolytic pathway. It can also be cleaved by PS1/ -secretase (at residues 731-732) and this cleavage promotes disassembly of adherens junctions. Clone 19A11 is an activating monoclonal antibody that stimulates adhesion of cells to E-cadherin substrate. It modifies the adhesive properties of E-cadherin and prevent the metastatic invasion of mouse lung cancer cells expressing human E-cadherin. This clone is shown to bind to the extracellular domain of E-cadherin near its primary adhesive motif: the strand swap dimer interface, which strengthens adhesion by stabilizing strand-swap dimers. (Ref.: Xie, B., et al. (2022). Proc. Natl. Acad. Sci. USA. 119 (32); e2204473119; Petrova, YI., et al. (2012). Mol. Biol. Cell. 23(11); 2092-2108; Marambaud, M., et al. (2002). EMBO J. 21(8); 1948-1956; Steinhausen, U., et al. (2001). J. Biol. Chem. 276(7); 4972-4980).

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable