MABN2547

Anti-VMAT1 Antibody, clone N440 21

clone N440/21, from mouse

• Synonym(s): Chromaffin granule amine transporter, Solute carrier family 18 member 1, Vesicular amine transporter 1, VAT1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N440/21, monoclonal
• species reactivity: mouse, rat
• packaging: antibody small pack of 25 μL
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_694694.1
• UniProt accession no.: Q8R090
• target post-translational modification: unmodified
• Gene Information: mouse ... Slc18A1(110877)

Description

General description

Chromaffin granule amine transporter (UniProt: Q8R090; also known as Solute carrier family 18 member 1, Vesicular amine transporter 1, VMAT1, VAT1) is encoded by the Scl19a1 (also known as Vmat1) gene (Gene ID: 110877) in murine species. Vesicular monoamine transporters (VMATs) are involved in presynaptic storage and release of neurotransmitters. They are located in the presynaptic region and are involved in the transport of monoamines into storage vesicle, which are released upon arrival of an action potential. Two structurally related, but pharmacologically distinct, VMATs have been described (VMAT1 and VMAT2) that are encoded by separate genes. VMAT1 is a multi-pass membrane acidic glycoprotein that is found in both large dense-core vesicles and in small synaptic vesicles. It s both C- and N-terminal regions are located in the cytosolic side of the vesicle. VMAT1 is detected in adrenal medulla, and brain (at protein level). In brain, specifically found in the medulla oblongata, pons, prefrontal cortex, striatum, dentate gyrus and hippocampus. VMAT1 is shown to play a key role in survival of hippocampal neurons and may contribute to neurocognitive deficits observed in neuropsychiatric disorders. (Ref.: Multani, PK., et al. (2013). Neurosci. 232; 32-44).

Specificity

Clone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.

Immunogen

Epitope: extracellular domain

Recombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).

Application

Anti-VMAT1, clone N440 21, Cat. No. MABN2547, is a highly specific mouse monoclonal antibody that targets Vesicular Monoamine Transporter 1 and has been tested for use in Western Blotting.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in rat cerebellum tissue lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected VMAT1 in rat cerebellum tissue lysate.

Target description

~93 kDa observed; 56.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.