MABN153

Anti-HuC/HuD Antibody, clone 15A7.1

clone 15A7.1, from mouse

• Synonym(s): ELAV-like protein 3, Hu-antigen C, HuC, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, ELAV-like protein 4, Hu-antigen D, HuD, Paraneoplastic encephalomyelitis antigen HuD
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 15A7.1, monoclonal
• species reactivity: human, rat, mouse
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001411
• UniProt accession no.: P26378; Q14576
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ELAVL4(1996)

Description

General description

Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding proteins that contain three RNA recognition motifs. RNA binding protein HuC promotes neuronal development in both the central and the peripheral nervous systems. HuC antibodies are used as markers for developing neurons. HuD may play a role in neuron-specific RNA processing, and be involved in maintaining the proliferative state of cells.

Immunogen

KLH-conjugated linear peptide corresponding to human HuC/HuD.

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected HuC/HuD in human brain and rat hippocampus tissues.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This HuC/HuD antibody is validated for use in WB & IHC for the detection of the HuC/HuD protein.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.

Target description

~ 40 kDa observed. An uncharacterized band may appear at ~45 kDa in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable