MABF3203

Anti-Tissue Factor/CD142 Antibody, clone HTF-1

• Synonym(s): Coagulation factor III, HTF1-7B8, TF, Thromboplastin
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: HTF-1, monoclonal
• mol wt: calculated mol wt 33 kDa; observed mol wt ~42-48 kDa
• purified by: using protein G
• species reactivity: human, bovine
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; dot blot: suitable; flow cytometry: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: NP_001171567
• UniProt accession no.: P13726
• storage temp.: -10 to -25°C
• Gene Information: human ... F3(2152)

Description

Specificity

Clone HTF-1 is a mouse monoclonal antibody that specifically detects Tissue Factor/CD142 protein.

Immunogen

Full length native (purified) human Tissue Factor.

Application

Immunofluorescence Microscopy

Tissue factor: Identification and characterization of cell types in human placentae. (Faulk, W.P., C.A. Labarrere, and S.D. Carson. (1990). Blood 76:86-96).

Immunofluorescent studies of tissue factor on U87MG cells: Evidence for non-uniform distribution. Carson, S.D., and S.J. Pirruccello. (1993). Blood Coagulation and Fibrinolysis 4:911-920).

Flow Cytometry

Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient. (Carson, S.D., S.J. Pirruccello, and W.D. Haire. (1990). Thrombos. Res. 59:159-170).

Immunohistochemistry

Participation of cell-mediated immunity in the deposition of fibrin in glomerulonephritis. (Neale, T.J., P.G. Tipping, S.D. Carson, and S.R. Holdsworth. (1988). Lancet, No. 8606, 2:421-424).

Tissue factor antigen in senile plaques of Alzheimer′s disease. (McComb, R.D., K.A. Miller, and S.D. Carson. (1991). Am. J. Path. 139:491-494).

Immunoaffinity purification of the tissue factor protein

An inhibitory monoclonal antibody against human tissue factor. (Carson, S.D., S.E. Ross, R. Bach, and A. Guha. (1987). Blood 70:490-493).

Quality Control Testing

Evaluated by Western Blotting in A-431cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Tissue Factor/CD142 in A-431 cell lysate.

Tested Applications

ELISA Analysis: A representative lot detected Tissue Factor/CD142 in ELISA applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).

Western Blotting Analysis: A representative lot detected Tissue Factor/CD142 in Western Blotting applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3). .

Dot Blot: A representative lot detected Tissue Factor/CD142 in Dot Blot applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).

Inhibition Assay: A representative lot detected Tissue Factor/CD142 in Inhibition applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Tissue factor (UniProt: P13726; also known as TF, Coagulation factor III, Thromboplastin, CD142) is encoded by the F3 gene (Gene ID: 2152) in human. Tissue factor (TF) is an integral membrane protein that serves as a high-affinity receptor and cofactor for factor VII/VIIa. It is expressed on perivascular cells and epithelial cells at organ and body surfaces where it forms a hemostatic barrier. It is also reported to provide hemostatic protection to brain, lung, and heart. In normal healthy individuals, levels of TF are very low. Cell-surface levels of TF do not display any directly correlation with its procoagulant activity indicating that TF exists on the cell surface as dormant (encrypted) and active (de-encrypted) form. This allows for a rapid hemostatic response without a need for transcriptional upregulation of the F3 gene. TF normally exists in a partially encrypted state and its de-encryption on circulating monocytes, platelets or endothelial cells by inflammatory mediators can lead to thrombosis. It initiates blood coagulation by forming a complex with circulating factor VII or VIIa and this complex activates factor IX and X by specific, limited proteolysis. TF expression by nonvascular cells plays an essential role in homeostasis; however, TF expression by vascular cells can induce life-threatening intravascular thrombosis in various diseases, such as sepsis, cancer, and atherosclerosis. Inhibition of TF-Factor VIIa activity is shown to reduce coagulation and mortality in animal models of sepsis. Many cancer cells express large amounts of TF, which contributes to tumor angiogenesis and growth. Cancer cells can release TF-positive microparticles into the circulation that can trigger venous thromboembolism. TF on circulating tumor cells can also coat cells with fibrin that traps them within the microvasculature and promote hematogenous metastasis. (Ref.: Carson, S.D., et al. (1987). Blood. 70(2):490-3; Caldwell, JA., et al. (2010). Lab. Invest. 90; 953-962; Kasthuri, RS., et al. (2009). J. Clin. Oncol. 27(29); 4834-4838; Mackman, N. (2004).

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable