MAB13446
Anti-TIMP-2 Antibody, clone 2TMP05
clone 2TMP05, Chemicon®, from mouse
Synonym(s):
Tissue Inhibitor of Metalloproteinase-2
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2TMP05, monoclonal
species reactivity
human, mouse, bovine, guinea pig, rabbit, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TIMP2(7077)
Specificity
MAB13446 recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinase-2 (TIMP-2). TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. CHEMICON MAB13446 may be used to evaluate the invasive nature of some tumor types and determine the tissue distribution of TIMPs in other tumors such as adenomas and also its normal tissue.
CELLULAR LOCALIZATION:
Cytoplasmic
CELLULAR LOCALIZATION:
Cytoplasmic
Immunogen
A synthetic peptide from the N-terminal region of human TIMP-2 protein.
Application
Detect TIMP-2 using this Anti-TIMP-2 Antibody, clone 2TMP05 validated for use in WB, IH(P).
Immunohistology (formalin/paraffin only): 2-4μg/ml 30 min at RT.
Western blotting: 1μg/ml for 2 hrs at RT.
(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.
Western blotting: 1μg/ml for 2 hrs at RT.
(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).
The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Linkage
Replaces: MAB13448
Physical form
Format: Purified
Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Storage and Stability
Antibody with sodium azide is stable for 12 months after date of receipt when stored at 2-8°C.
Analysis Note
Control
POSITIVE CONTROL:
Pancreatic or colon carcinoma
POSITIVE CONTROL:
Pancreatic or colon carcinoma
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Davide Visigalli et al.
BMC cancer, 9, 59-59 (2009-02-20)
The COOH terminal peptide of Pro-collagen type I (PICP, also called C3) is chemotactic for endothelial melanoma and breast cancer cells. PICP induces the expression of Metalloproteinases-2 and -9, of Vascular endothelial growth factor and of the chemokine CXCL-12 receptor
Effect of doxycycline on postoperative scarring after trabeculectomy in an experimental rabbit model.
Emine Sen,Melike Balikoglu-Yilmaz,Sibel Bozdag-Pehlivan,Nuran Sungu,Fatma Nur Aksakal et al.
Journal of Ocular Pharmacology and Therapeutics null
Jaqueline J Muniz et al.
Canadian journal of physiology and pharmacology, 96(3), 266-274 (2017-08-19)
We evaluated the effects of ethanol consumption on the mitogen-activated protein kinases (MAPK) and metalloproteinases (MMP) pathways in the rat cavernosal smooth muscle (CSM). Male Wistar rats were treated with ethanol (20% v/v) for 6 weeks. Quantitative real-time polymerase chain
Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs.
Linard, C; Busson, E; Holler, V; Strup-Perrot, C; Lacave-Lapalun, JV; Lhomme, B; Prat et al.
Stem Cells Translational Medicine null
Angiotensin-II and rosuvastatin influence matrix remodeling in human mesangial cells via metalloproteinase modulation.
Solini A, Rossi C, Santini E, Madec S, Salvati A, Ferrannini E.
Journal of Hypertension null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service