Skip to Content
Merck
All Photos(1)

Documents

CC324-1

Sigma-Aldrich

PhotoGel-LAP, Methacrylated Gelatin Hydrogel Kit

Photocrosslinked gelatin hydrogel (CC324 Kit Component 1)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

form

lyophilized powder

application(s)

cell analysis

General description

3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. The PhotoGel-LAP, Methacrylated Gelatin Hydrogel Kit (CC324: CC324-1, CC324-2) is based upon purified porcine gelatin methacrylate (GelMA), which when photocrosslinked provides a native-like 3D environment for cells. Gelatin derived from denatured collagen retains many natural cell binding motifs such as RGD and MMP sites. In addition to porcine gelatin methacrylate, the kit includes the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). Gelatin methacrylate is produced from porcine, type A, 300 bloom gelatin. Gelatin macromers containing primary amino groups were reacted with methacrylic anhydride (MA) to add methacrylate pendant groups. The gelatin methacrylate achieves a degree of substitution of >70% for maximum crosslinking and range of stiffness.PhotoGel is a registered trademark of Advanced BioMatrix, Inc.

Application

Photocrosslinking is initiated using a 405 nm blue light source.

Packaging

1 Kit

Components

CC324-1 (Store at 2-8°C): LAP Photoinitiator, 1 X 100 mg (CS226439).
Note: The complete PhotoGel-LAP, Methacrylated Gelatin Hydrogel Kit (CC324) consists of CC324-1 and CC324-2 sold seperately.

Quality

Appearance: Lyophilized powderGelatin Content: ≥ 1 gramSterility (USP modified): No GrowthpH: 6.0-8.0Grafting Efficiency: ≥ 75%Cell Compatibility: >70% Cell Viability

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Photocrosslinkable Hydrogels for 3D Cell Culture and Tissue Engineering

Methacrylated collagen, hyaluronic acid, and gelatin (GelMA) hydrogels can be crosslinked with light and photoinitiators (Irgacure/LAP/Ruthenium), used as 3D cell culture scaffolds and bioinks for bioprinting.

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service