Skip to Content
Merck
All Photos(1)

Key Documents

ABS190

Sigma-Aldrich

Anti-PIP5K-Type 1 gamma Antibody

from rabbit

Synonym(s):

Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma, PIP5K1-gamma, PtdIns(4)P-5-kinase 1 gamma, Phosphatidylinositol 4-phosphate 5-kinase type I gamma, PIP5KIgamma

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_001140159

UniProt accession no.

O70161

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Pip5K1C(18717)

General description

Phosphatidylinositol-4-phosphate 5-kinase 1 gamma (PIP5K type 1 gamma; PIP5K1γ) is a ubiquitous protein which is enriched in focal adhesion plaques. It is expressed in relatively high levels in the brain, particularly at synaptic sites. PIP5K type 1 gamma is activated by talin, and in turn phosphorylates phosphatidylinositol 4-phosphate to form phosphatidylinositol 4,5-bisphosphate, the precursor to the second messenger phosphatidylinositol 3,4,5-trisphosphate. It indirectly influences vesicle transport, cell adhesion, and cell migration, among other processes. Defects in PIP5K type 1 gamma may contribute to lethal congenital arthrogryposis.

Immunogen

recombinant protein corresponding to mouse PIP5K-type 1 gamma.

Application

Anti-PIP5K-Type 1 gamma Antibody detects level of PIP5K-Type 1 gamma & has been published & validated for use in Western Blotting & IHC.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PIP5K-Type 1 gamma in rat brain tissues (Wenk, M. R., et al. 2001. Neuron. 32(1):79-88.).
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 mg/mL of this antibody detected PIP5K-Type 1 gamma in 10 mg of mouse brain tissue lysate.

Target description

~90 kDa observed. The calculated molecular weight of PIP5K Type-1 gamma is 69-72 kDa, but has been observed at ~90 kDa in Western Blot (Volpicelli-Daley, L.A., et al. (2010). 285(37):28708-28714.).

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

M R Wenk et al.
Neuron, 32(1), 79-88 (2001-10-18)
Disruption of the presynaptically enriched polyphosphoinositide phosphatase synaptojanin 1 leads to an increase of clathrin-coated intermediates and of polymerized actin at endocytic zones of nerve terminals. These changes correlate with elevated levels of PI(4,5)P(2) in neurons. We report that phosphatidylinositol

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service