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8.05740

Sigma-Aldrich

2-Mercaptoethanol

for synthesis

Synonym(s):

Thioethylene glycol, Thioglycol, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
Beilstein:
773648
MDL number:
MFCD00004890
UNSPSC Code:
12352104
EC Index Number:
200-464-6
NACRES:
NA.22

vapor density

2.69 (vs air)

Quality Level

200

vapor pressure

1 mmHg ( 20 °C)

Assay

≥99% (GC)

form

liquid

autoignition temp.

295 °C

potency

98-162 mg/kg LD50, oral (Rat)
112-224 mg/kg LD50, skin (Rabbit)

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L in H2O)

bp

157 °C (lit.)

mp

<-50 °C

transition temp

flash point 70.5 °C

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-30°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

165.2 °F - closed cup

Flash Point(C)

74 °C - closed cup

Certificates of Analysis (COA)

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Georg Michlits et al.
Nature methods, 17(7), 708-716 (2020-06-10)
CRISPR-Cas9 screens have emerged as a transformative approach to systematically probe gene functions. The quality and success of these screens depends on the frequencies of loss-of-function alleles, particularly in negative-selection screens widely applied for probing essential genes. Using optimized screening

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