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Key Documents

70955

Millipore

5% Alkali-soluble Casein

Unique blocking reagent for ultra-low background

Synonym(s):

blocking buffer, immunodetection blocking buffer

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About This Item

UNSPSC Code:
41105329

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

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General description

Alkali-Soluble Casein is a superior blocking reagent for Western blot applications. This preparation minimizes non-specific background relative to other blocking reagents, such as BSA, gelatin, or non-fat milk. Alkali-Soluble Casein is effective in colorimetric and chemiluminescent detection protocols. Note that due to its high coating properties, detection sensitivity for some antibodies may be lower than with other blocking reagents under the same conditions (although the background is also reduced); the concentration can be optimized for maximal signal:noise ratios. Supplied as a 5% w/v solution; recommended working concentration range is 0.251%.
Unique blocking reagent for ultra-low background

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317 - H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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