475911
Myeloperoxidase, Human Polymorphonuclear Leukocytes
A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.
Synonym(s):
Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
Sign Into View Organizational & Contract Pricing
Select a Size
All Photos(1)
Select a Size
Change View
About This Item
Recommended Products
biological source
human
Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized
specific activity
150-200 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble (volume is lot-specific and can be found on the product label)
shipped in
wet ice
storage temp.
−20°C
General description
Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
Physical form
Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Preparation Note
Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
Other Notes
Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service