444805

Membrane Traffic Inhibitor, A5

The Membrane Traffic Inhibitor, A5 controls the biological activity of adaptor complex AP-1.

• Synonym(s): Membrane Traffic Inhibitor, A5, 1-(4-(4-(E-2-Methyl-2-butenyl)piperazinyl)phenyl)ethanone, HCl, 5431942, A5
• Empirical Formula (Hill Notation): C₁₇H₂₄N₂O · xHCl
• Molecular Weight: 272.39 (free base basis)
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: pale yellow
• solubility: water: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC₅₀ = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Duncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA104, 6235.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable