400010

Caspase-1 Inhibitor I

The Caspase-1 Inhibitor I, also referenced under CAS 143313-51-3, controls the biological activity of Caspase-1. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Caspase-1 Inhibitor I, IL-1β Converting Enzyme (ICE) Inhibitor I, Ac-YVAD-CHO, L-709049
• Empirical Formula (Hill Notation): C₂₃H₃₂N₄O₈
• CAS Number: 143313-51-3
• Molecular Weight: 492.52
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMSO: 5 mg/mL; water: soluble
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C23H32N4O8/c1-12(2)20(23(35)27-21(33)13(3)24-16(11-28)10-19(31)32)26-22(34)18(25-14(4)29)9-15-5-7-17(30)8-6-15/h5-8,11-13,16,18,20,24,30H,9-10H2,1-4H3,(H,25,29)(H,26,34)(H,31,32)(H,27,33,35)/t13-,16-,18-,20-/m0/s1
• InChI key: KQCBPHUOVBKBPQ-SJVNDZIOSA-N

Description

General description

A potent, specific, and reversible inhibitor of caspase-1 (K_i = 200 pM for human recombinant caspase-1), caspase-4, and caspase-5. Strongly inhibits anti-APO-1 induced apoptosis in L929-APO-1 cells.

Interleukin-1β converting enzyme (ICE) is a cysteine protease that cleaves the IL-1β precursor to mature IL-1β. ICE inhibitor I is a potent, specific, and reversible inhibitor of ICE (K_i = 200 pM for human recombinant ICE). Strongly inhibits anti-APO-1 induced apoptosis in L929-APO-1 cells.

Biochem/physiol Actions

Cell permeable: no

Primary Target
caspase-1, human recombinant

Product does not compete with ATP.

Reversible: yes

Target K_i: 200 pM for human recombinant caspase-1

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Tyr-Val-Ala-Asp-CHO

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608.
Hilbi, H., et al. 1998. J. Biol. Chem. 273, 32895.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Los, M., et al. 1995. Nature375, 81.
Walker, N.P.C., et al. 1994. Cell78, 343.
Wilson, K.P., et al. 1994. Nature370, 270.
Thornberry, N.A., et al. 1992. Nature356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable