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Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

clone PY20, Upstate®, from mouse

Synonym(s):

PY20, anti-phosphotyrosine antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PY20, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG2b

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PID1(55022)

General description

Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.

Specificity

Recognizes proteins containing phosphotyrosine residues, regardless of origin.

Immunogen

Epitope: phosphotyrosine
Phosphotyrosine conjugated to carrier protein

Application

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Quality

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

Target description

Varies

Physical form

100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
Protein A purified

Storage and Stability

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Becky K Brisson et al.
PloS one, 7(9), e45588-e45588 (2012-10-03)
Insulin-like growth factor-I (IGF-I) is an essential growth factor that regulates the processes necessary for cell proliferation, differentiation, and survival. The Igf1 gene encodes mature IGF-I and a carboxy-terminal extension called the E-peptide. In rodents, alternative splicing and post-translational processing

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