1.52358

Chromolith^® WP 300 Protein A

L × I.D. 25 mm × 2 mm HPLC column

• Synonym(s): Chromolith^® WP 300 Protein A 25-2 HPLC column

Properties

• Quality Level: 100
• feature: endcapped: no
• parameter: 2-45 °C temp. range; 200 bar pressure
• technique(s): HPLC: suitable
• column L × I.D.: 25 mm × 2 mm
• matrix: monolithic silica
• matrix active group: Protein A bonding phase
• pore size: 2 μm macroporosity; 30 nm mesoporosity
• operating pH: 1.5-7.5
• storage temp.: 2-8°C

Description

General description

Affinity chromatography is a selective technique that takes advantage of specific molecular interactions, for example, antigen and antibody. The Chromolith^® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith^® WP 300 Protein A, 25-2 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:

• Rapid and accurate titer determination across a wide range of injection volumes
• Consistent binding efficiencies even at high flow rates
• Expanded column lifetimes

Learn more:
Flyer: Rapid and Robust Antibody Quantitation - Chromolith^® WP 300 Protein A HPLC columns

Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany