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Millipore

Rambach Agar

Ready-to-Use Chromogenic Culture Medium for the Isolation of Salmonella sp. in 90mm plates

Synonym(s):

Chromogenic Salmonella Agar, Salmonella Isolation Agar acc RAMBACH

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About This Item

UNSPSC Code:
41106217
NACRES:
NA.74

Agency

ISO 6579

Quality Level

300

form

(Solid in plate)

shelf life

4 mo.

feature

ready-to-use

packaging

pkg of (90 mm plate (20 or 100 plates per pack))

pH

7.3 ( in H2O)

application(s)

food and beverages
industrial qc
microbiology

storage temp.

2-8°C

suitability

Salmonella spp.

General description

Rambach Agar plates are standard 90mm stackable specially designed plates that help increase safety during handling, transport, and incubation. Rambach agar is a selective media for isolation and differentiation of Salmonella sp. from Proteus sp. and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella sp. and is utilized in these media.

Application

Rambach agar is a selective media for isolation and differentiation of Salmonella sp. from food and animal feed, water, and other materials.

Analysis Note

The nutritive substrates in the RAMBACH Agar enable Enterobacteriaceae to multiply readily. Sodium desoxycholate inhibits the accompanying Gram-positive flora. RAMBACH Agar enables species of Salmonella to be differentiated unambiguously from other bacteria by means of a new procedure, for which a patent application has been submitted. This is made possible by adding propylene glycol to the culture medium. Salmonellae form acid with propylene glycol, so that, in combination with a pH indicator, the colonies have a characteristic red colour. In order to differentiate coliforms from Salmonellae, the medium contains a chromogene indicating the presence of β-galactosidase splitting, a characteristic for coliforms. Coliform microorganisms grow as blue-green or blueviolet colonies. Other Enterobacteriaceae and Gram-negative
bacteria, such as Proteus, Pseudomonas, Shigella, S. typhi and S. parathyphi A grow as colourless-yellow colonies.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Procedure for Salmonella Detection According to EN ISO 6579-1

ISO published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in products intended for human consumption, animal feed, and environmental samples in food production and handling.

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