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129935

Sigma-Aldrich

Actinomycin D, 7-Amino-

7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.

Synonym(s):

Actinomycin D, 7-Amino-, 7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin

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About This Item

Empirical Formula (Hill Notation):
C62H87N13O16
CAS Number:
7240-37-1
Molecular Weight:
1270.43
MDL number:
MFCD00077295
UNSPSC Code:
12352200

Quality Level

100

Assay

≥85% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

red-purple

input

sample type intact cells

solubility

DMSO: 20 mg/mL
ethanol: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)

InChI key

YXHLJMWYDTXDHS-UHFFFAOYSA-N

General description

7-Aminoactinomycin D is a membrane impermeable fluorescent DNA intercalator. Like its parent compound, Actinomycin D (Cat. No. 114666), 7-AAD has antibacterial properties and growth inhibitory activity against certain leukemias and sarcomas. Useful for flow cytometry and fluorescence microscopy. Used for staining nonviable cells and for identifying apoptotic cells. Compatible with FITC and PE cell surface staining. The absorption/emission maxima are as follows: unbound (ex. 500 nm, em. 672 nm) and DNA bound (ex. 555 nm, em. 655 nm). λmax 528 nm ± 2; εmax = 22,700 ± 5,000 M-1 cm-1
7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Fluorescent excitation and emission maxima are 500 and 672 nm, respectively. Complexes with DNA and absorbs at 555 nm and emits at 655 nm. Useful in distinguishing early apoptotic cells, which have lost membrane integrity, from apoptotic and live cells. Has been used in DNA analysis and as a probe for chromosome structure and function.

Biochem/physiol Actions

Primary Target
leukemias and sarcomas

Warning

Toxicity: Highly Toxic (H)

Preparation Note

Further dilute in aqueous buffers just prior to use.

Reconstitution

Following reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Schmid, I., et al. 1994. J. Immunol. Methods 170, 145.
Berg, R.J., et al. 1993. Carcinogenesis14, 103.
Chen, F.M. 1992. Biochemistry31, 6223.
Schmid, I., et al. 1992. Cytometry13, 204.
Toba, K., et al. 1992. Cytometry13, 60.
Sengupta, S.K., et al. 1975. J. Med. Chem.18, 1175.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

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