1.09878

Modified Semi-solid RAPPAPORT-VASSILIADIS medium (base)

pkg of 500 g, ISO 6579, for Salmonella spp.

• Synonym(s): MSRV medium (base)
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: ISO 6579
• Quality Level: 200
• form: powder (dehydrated (DCM))
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^®
• technique(s): microbiological culture: suitable
• pH: 5.1-5.4 (25 °C, 31.8 g/L in H₂O)
• solubility: 31.6 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Salmonella spp.

Description

General description

Modified Semi-solid RAPPAPORT-VASSILIADIS medium (base) is a semi-solid modification of Rappport-Vassiliadis enrichment broth. It is a rapid and sensitive medium for the isolation of Salmonella spp. and can be seeded directly after pre-enrichment. Its efficiency is due to the ability of salmonellae to migrate into the semi-solid medium, thereby forming opaque halos of growth. The selective agents like magnesium chloride, malachite green, and novobiocin, along with the enhanced incubation temperature of 41.5 °C, inhibit the motility of other microorganisms. This medium is not suitable for detecting non-motile Salmonellae since the detection principle is based on motility.

Application

Modified Semi-solid RAPPAPORT-VASSILIADIS medium (base) is suitable for the selective enrichment and presumptive detection of Salmonella from food and animal feed, animal feces, and environmental samples from the primary production stage.

Analysis Note

Appearance (clearness): clear
Appearance (color): blue
pH-value (25 °C): 5.1 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): Grey-white, turbid zone extending out from inoculated drop. After 24–48 h, the turbid zone will be (almost) fully migrated over the plate.
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): Grey-white, turbid zone extending out from inoculated drop. After 24–48 h, the turbid zone will be (almost) fully migrated over the plate.
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): Possible growth at the place of the inoculated drop without a turbid zone.
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): Possible growth at the place of the inoculated drop without a turbid zone.
Growth (Citrobacter freundii ATCC 8090): no growth or possible growth at the place of the inoculated drop without a turbid zone.
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): no growth
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): no growth
Incubation:
2 x 24 ± 3 hours at 41,5 ± 1 °C aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1