1.00465

GranuCult^® prime DG 18 (Dichloran glycerol chloramphenicol) agar (base)

ISO 21527-2:2017, FDA-BAM, for molds, suitable for microbiology

• Synonym(s): DG 18 agar (base), Dichloran glycerol chloramphenicol agar Base
• UNSPSC Code: 41106213

Properties

• Agency: APHA; FDA-BAM; ISO 21527-2:2017
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 5.4-5.8 (25 °C in H₂O)
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: molds; yeasts

Description

General description

GranuCult^® prime DG 18 (Dichloran glycerol chloramphenicol agar Base (acc. ISO 21527 and FDA-BAM) is recommended for the enumeration of xerophilic molds in dried and semi-dried foods. Reduced water activity from approx. 0.99 to 0.95 with 18 % (w/w) glycerol, and the addition of chloramphenicol prevents the growth of bacteria. Dichloran inhibits the rapid spreading of mucoraceous fungi and restricts colony sizes of other genera, easing the colony count. The enzymatic digest of casein is a nitrogen source containing an elevated level of free amino acids. Glucose provides an energy source for the growth, while agar is the solidifying agent.

Application

GranuCult^® prime DG-18 agar (base) is suitable for the enumeration of xerophilic molds in dried and semi-dried foods, such as dried fruits, meat and fish products, spices, confectionery, cereals, and nuts.

Features and Benefits

• GranuCult^® offers superior granulated culture media.
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions.
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (colour): yellowish
pH-value (25 °C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 50 %
Inoculum on reference medium (Wallemia sebi ATCC 42694 (WDCM00182)):
Colony count (Wallemia sebi ATCC 42694 (WDCM00182)):
Recovery on test medium (Wallemia sebi ATCC 42694 (WDCM00182)): ≥ 50 %
Inoculum on reference medium (Aspergillus caesiellus ATCC 42693 (WDCM00183)):
Colony count (Aspergillus caesiellus ATCC 42693 (WDCM00183)):
Recovery on test medium (Aspergillus caesiellus ATCC 42693 (WDCM00183)): ≥ 50 %
Inoculum on reference medium (Eurotium rubrum ATCC 42690 (WDCM00183)):
Colony count (Eurotium rubrum ATCC 42690 (WDCM00183)):
Recovery on test medium (Eurotium rubrum ATCC 42690 (WDCM00183)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no growth
Growth (Mucor racemosus ATCC 42647): good to very good
Growth (Penicillium cyclopium CMI 19759): good to very good
Growth (Rhizopus stolonifer ATCC 15441): good to very good
Incubation: 5 days at 25 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD dextrose agar

Other Notes

• The prepared medium is clear to slightly opalescent and yellowish-brown.
• The self-prepared plates can be stored at +2 °C to +8 °C in the dark and protected against evaporation for up to 7 days.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3