07-307
Anti-phospho-STAT1 (Tyr701) Antibody
Upstate®, from rabbit
Synonym(s):
Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1, signal transducer and activator of transcription 1, 91kD, signal transducer and activator of transcription 1, 91kDa, signal transducer and activator of tr
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, chicken, human, rat
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pTyr701)
Gene Information
human ... STAT1(6772)
General description
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
Specificity
Other species cross-reactivity is unknown.
Phosphorylated STAT1
Immunogen
Epitope: Tyr701
KLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine.
Application
Anti-phospho-STAT1 (Tyr701) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-STAT1 (Tyr701) also known as Transcription factor ISGF-3 components p91/p84 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated by immunoblot in RIPA lysates from CNTF-treated SK-N-MC cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
Target description
92 kDa
Physical form
Immunoaffinity Chromatography
Immunoaffinity purified IgG in 0.02 M PBS containing 0.1% Sodium Azide + 50% Glycerol and 5 mg/mL BSA.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Control
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Ana Maria Low-Calle et al.
Virus research, 180, 49-58 (2014-01-01)
Herpes simplex virus type 1 (HSV-1) causes a lytic infection in epithelial cells before being captured and moved via retrograde axonal transport to the nuclei of the sensory neurons of the trigeminal ganglion or dorsal root, where it establishes a
Induction of type I interferons by a novel porcine reproductive and respiratory syndrome virus isolate.
Nan, Yuchen, et al.
Virology, 432, 261-270 (2012)
STAT2 deficiency and susceptibility to viral illness in humans.
Hambleton, S; Goodbourn, S; Young, DF; Dickinson, P; Mohamad, SM; Valappil, M; McGovern et al.
Proceedings of the National Academy of Sciences of the USA null
Precision-cut slice cultures of tumors from MMTV-neu mice for the study of the ex vivo response to cytokines and cytotoxic drugs.
Nirmala Parajuli, Wolfgang Doppler
In Vitro Cellular & Developmental Biology. Animal null
Hepatitis C virus core protein differently regulates the JAK-STAT signaling pathway under interleukin-6 and interferon-gamma stimuli.
Hosui, Atsushi, et al.
The Journal of Biological Chemistry, 278, 28562-28571 (2003)
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