810603C

16:0-10 Doxyl PC

Avanti Polar Lipids 810603C

• Synonym(s): 1-palmitoyl-2-stearoyl-(10-doxyl)-sn-glycero-3-phosphocholine
• Empirical Formula (Hill Notation): C₄₆H₉₀N₂O₁₀P
• CAS Number: 188004-25-3
• Molecular Weight: 862.19
• UNSPSC Code: 41141825

Properties

• Assay: >99% (TLC)
• form: liquid
• packaging: pkg of 1 × 1 mL (810603C-1mg)
• manufacturer/tradename: Avanti Polar Lipids 810603C
• concentration: 1 mg/mL (810603C-1mg)
• lipid type: ESR probes; phospholipids
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.

Phosphocholine is considered as a precursor molecule. It is formed during the breakdown of phosphatidylcholine metabolism.

Application

16:0-10 Doxyl PC may be used in vesicles for quenching PT-(1–46)F4W fluorescence. It may also be used in vesicles to check the fluorescence emission spectra in order to measure the penetration depth of the peptides′ tryptophan residues in lipid bilayer.

Biochem/physiol Actions

Phosphatidylcholine (PC) lowers the levels of cholesterol and triglycerides.

Packaging

5 mL Clear Glass Sealed Ampule (810603C-1mg)

Preparation Note

Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412
• Precautionary Statements: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash