Skip to Content
Merck

PCR in as Little as 15 Minutes!

KOD One™ PCR Master Mix for
Ultra-Fast, High Fidelity PCR

KOD One™ PCR Master Mix

KOD One™ PCR Master Mix is the right choice for ultra-fast PCR. It offers the fastest elongation rates available for high fidelity polymerases without compromising on the yield and specificity. This convenient, premixed, ready-to-use solution offers an elongation rate of up to 5 seconds per kb and exhibits approximately 80-fold higher fidelity than Taq DNA polymerase. Additionally, this flexible master mix will easily fit right into your PCR workflow with its ability to amplify directly from crude samples and use primers containing both uracil and inosine. Choose KOD One™ PCR Master Mix and efficiently amplify your samples with confidence.

KOD One™ PCR Master Mix

Benefits

The KOD One™ PCR Master Mix formulation offers the following advanced features:

  • Ready-to-Use
  • Added Flexibility
  • High Fidelity
  • High Efficiency
  • Crude Sample PCR
  • High Specificity
  • Primers with inosine (dI) and uracil (dU)

Applications

This product has a variety of applications such as:

  • DNA/cDNA amplification
  • Direct PCR
  • Colony PCR
  • NGS library amplification
  • PCR amplification to detect site-directed mutagenesis

PCR Master Mix and Polymerase Cross Reference Guide 

KOD One™ PCR Master Mix Customer Testimonials

Aleksandar Vještica
Assistant Professor

Organization: University of Lausanne
Field: Academia / University

"Thanks to its high processivity, KOD One™ Master Mix allowed us to quickly clone plasmids using only overlapping PCRs. This approach did not require restriction enzyme digestions of cloning inserts and thus considerably simplified the cloning protocol and saved us time. "

Review date: 3 December 2021
KOD One™ PCR Master Mix

Blanco Lago Solis
Ph.D. Student

Organization: University of Geneva
Field: Academia / University

"I successfully amplified a 14kb fragment in 2h15 vs 4h30 with Phusion or Q5."

KOD One™ PCR Master Mix

Maria Cristina Gambetta
Assistant Professor

Organization: University of Lausanne
Field: Academia / University

"I was caught red-handed in my impatience. I asked around and indeed got an aliquot of KOD One™ master mix promising to give it back. It worked wonderfully! I guess this really is a miraculous polymerase. So, we went ahead and ordered master mix to not waste time, as experiments are urgent."

Review date: 16 December 2021
KOD One™ PCR Master Mix

Dr. Michael Taschner
Senior Scientist

Organization: University of Lausanne
Field: Academia / University

"I received a free sample of KOD One™ PCR master mix to test. I was amazed by the speed, processivity, and accuracy of PCRs carried out on complicated templates. No other product I used in the past was able to amplify a product of >12 kb in record time from ‘quick-and-dirty’ yeast genomic DNA (purified according to [Lōoke et al, BioTechniques, 2011]. I fully recommend this product for difficult PCR cases."

Review date: 17 December 2021
KOD One™ PCR Master Mix

Related Applications
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
qPCR
Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
Advanced Gene Editing
An overview of CRISPR/Cas9 and ZFN principles and techniques, and their implementation in targeted genome editing and genome engineering for research and therapeutic applications.
DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Next Gen Sequencing
Next-Generation Sequencing (NGS) reagents and resources for massively parallel sequencing applications.
Nucleic Acid Labeling & Detection
DNA and RNA nucleic acid labeling and detection methods and applications for northern blot, Southern blot, and additional nucleic acid detection assays.
Sequencing
DNA sequencing reagents and tools for Sanger sequencing methods, DNA primers, probes, and additional nucleic acid sequencing applications.
Related Product Categories
PCR Reagents & Kits
Master PCR for Discovery: Amplify DNA exponentially with our reagents, supporting gene analysis, sequencing, mutagenesis, and more.
Whole Genome Amplification
Whole Genome Amplification (WGA) kits, reagents, and protocols to support your WGA workflow.
PCR Supplies & Equipment
PCR tubes, tips, filter tips, plates, plate sealers, PCR thermocyclers and additional consumables to support your PCR workflow.
Molecular Cloning & Protein Expression
Molecular cloning and protein expression methods and protocols for reliable cloning and robust protein expression from insect, bacteria, and mammalian protein expression vectors.
Roche® Life Science Products
Roche Life Science Products - As the exclusive distributor of Roche products and biochemical reagents, we are committed to enabling your next breakthrough by providing dependable reagents that can quickly deliver high-quality, reproducible results.
Custom & Predesigned DNA Oligos and qPCR Probes
Discover our custom DNA oligos portfolio including primers, qPCR probes, high-throughput DNA in plates, and next-generation sequencing (NGS) adapters available in multiple formats that comply with ISO 9001 and ISO 13485 quality systems for research and commercial applications.
gDNA Purification
High yield, high quality DNA extraction and purification kits tailored to a variety of common and challenging sample types including cell culture, mammalian tissue, blood, viruses, bacteria, plant tissue, soil, water, urine, stool, PCR reactions, and agarose gel extractions.
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?