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GE17-5158-01

HiTrap® Q XL

Cytiva 17-5158-01

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

quaternary amine

manufacturer/tradename

Cytiva 17-5158-01

matrix

6% cross-linked agarose with dextran surface extenders

cleaning

2-14

working range

2-12

General description

HiTrap® Q XL are strong anion exchangers prepacked with Q Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Application

Q Sepharose XL and SP Sepharose XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Features and Benefits

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page covers how and when to use Sepharose XL media for purification of proteins.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service