GE17-1408-01
HiTrap® Desalting Columns
Cytiva 17-1408-01, pack of 5 × 5 mL
Synonym(s):
Purification Columns
About This Item
Recommended Products
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-1408-01
storage condition
dry media
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
cross-linked dextran
particle size
>20 μm (dry)
cleaning
2-13
working range
2-13
suitability
suitable for bioprocess medium
Related Categories
General description
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
Application
Features and Benefits
- Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
- Replaces dialysis as the method of choice for desalting.
- Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocols
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to prepare samples for purification with affinity chromatography.
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