OP97
Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)
liquid, clone 22C2, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
22C2, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MTOR(2475)
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SpAG8 mouse myeloma cells. Recognizes the ~290 kDa mTOR/FRAP protein.
Recognizes the ~290 kDa mTOR protein in HEK293 and Jurkat cells.
This Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) is validated for use in Immunoblotting, Immunoprecipitation for the detection of mTOR/FRAP (Ab-2).
Immunogen
Human
a synthetic peptide corresponding to amino acids 230-240 of human TOR
Application
Immunoblotting (1.7 g/ml, see application references)
Immunoprecipitation (1-10 g/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Positive Control
Jurkat or HeLa cells
Jurkat or HeLa cells
Other Notes
Kimura N et al. 2003. Genes Cells8, 65.
Sekulic A et al. 2000. Cancer Res.60, 3504.
Hosoi, H. 1999. Cancer Res.59, 886.
Alarcon, C.M., et al. 1996. Genes Dev.10, 279.
Freeman, K., and Livi, G.P. 1996. Gene172, 143.
Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
Cardenas, M.E., and Heitman, J. 1995. EMBO J.14, 5892.
Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem.270, 27531.
Sabatini, D.M., et al. 1995. J. Biol. Chem.270, 20875.
Zheng, X.F., et al. 1995. Cell82, 121.
Brown, E.J., et al. 1994. Nature369, 756.
Sabatini, D.M., et al. 1994. Cell78, 35.
Stan, R., et al. 1994. J. Biol. Chem.269, 32027.
Kunz, J., et al. 1993. Cell73, 585.
Heitman, J., et al. 1991. Science253, 905.
Sekulic A et al. 2000. Cancer Res.60, 3504.
Hosoi, H. 1999. Cancer Res.59, 886.
Alarcon, C.M., et al. 1996. Genes Dev.10, 279.
Freeman, K., and Livi, G.P. 1996. Gene172, 143.
Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
Cardenas, M.E., and Heitman, J. 1995. EMBO J.14, 5892.
Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem.270, 27531.
Sabatini, D.M., et al. 1995. J. Biol. Chem.270, 20875.
Zheng, X.F., et al. 1995. Cell82, 121.
Brown, E.J., et al. 1994. Nature369, 756.
Sabatini, D.M., et al. 1994. Cell78, 35.
Stan, R., et al. 1994. J. Biol. Chem.269, 32027.
Kunz, J., et al. 1993. Cell73, 585.
Heitman, J., et al. 1991. Science253, 905.
The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M C Lorenz et al.
The Journal of biological chemistry, 270(46), 27531-27537 (1995-11-17)
The antifungal, immunosuppressive compound rapamycin arrests the cell cycle in G1 in both yeast cells and T-lymphocytes. Previous genetic studies in yeast identified mutations in three genes, FPR1 (FKBP12), TOR1, and TOR2, which confer rapamycin resistance, and genetic findings implicated
R Stan et al.
The Journal of biological chemistry, 269(51), 32027-32030 (1994-12-23)
The yeast TOR1 and TOR2 proteins were previously discovered as putative targets of the immunosuppressive drug rapamycin. Although their cellular function is unknown, they are predicted to be at least 215 kDa in size and possess a C-terminal phosphatidylinositol (PI)
J Kunz et al.
Cell, 73(3), 585-596 (1993-05-07)
The yeast TOR2 gene encodes an essential 282 kd phosphatidylinositol (PI) 3-kinase homolog. TOR2 is related to the catalytic subunit of bovine PI 3-kinase and to yeast VPS34, a vacuolar sorting protein also shown to have PI 3-kinase activity. The
C M Alarcon et al.
Genes & development, 10(3), 279-288 (1996-02-01)
In complex with the prolyl isomerase FKBP12, the natural product rapamycin blocks signal transduction in organisms as diverse as yeast and man. The yeast targets of FKBP12-rapamycin, TOR1 and TOR2, are large proteins with homology to lipid and protein kinases.
K Freeman et al.
Gene, 172(1), 143-147 (1996-06-12)
The TOR genes were first identified in Saccharomyces cerevisiae by the isolation of mutants which exhibit dominant resistance to the immunosuppressive and antifungal drug rapamycin (Rm). The originally characterized Rm-resistant (RmR) TOR1-1 and TOR2-1 alleles contain an Arg in place
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