G006369

HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL^™ LC-PAH after SPE using Supelclean^™ EZ-POP NP

application for SPE, application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable; solid phase extraction (SPE): suitable
• Testparameter: sample preparation: SPE (Solid Phase Extraction); sample/matrix: Canola oil spiked with PAHs at 10 ng/g; SPE tube/cartridge: Supelclean^™ EZ-POP NP, 12 mL, pk of 20 (54341-U); condition: 10 mL acetone, dry under 10-15" mercury for 10 minutes; sample addition: 0.5 mL weighed accurately onto cartridge; elution: 15 mL acetonitrile; eluate post-treatment: concentrate to final volume of 0.5mL at 40 °C under nitrogen; column: SUPELCOSIL^™ LC-PAH 25 cm x 4.6 mm I.D., 5 μm particles (58229); mobile phase: [A] water [B] acetonitrile; gradient: 40% B held for 5 min; to 100% B in 15 min; held at 100% B for 12 min; flow rate: 2.4 mL/min; pressure: 2790 psi at start (192 bar); column temp.: 25 °C; detector: Fluorescence, programmed.
• Eignung: application for HPLC; application for SPE
• Anwendung(en): food and beverages

Beschreibung

Hinweis zur Analyse

PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying [1]. In this application, EZ-POP NP SPE is used to extract PAHs from soybean oil. The 16 PAHs designated in many US EPA methods (less acenaphthylene) were analyzed by HPLC-FLD after extraction from a spiked sample of oil.

Sonstige Hinweise

Detector: Naphthalene, Acenaphthene, Fluorene - 225nm/320nm; Phenanthrene, Anthracene - 250nm/368nm; Fluoranthene, Pyrene - 237nm/440nm; Benzo[a]anthracene, Chrysene - 265nm/380nm; Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[a]pyrene, Dibenzo[a,h]anthracene - 280nm/420nm; Benzo[g,h,i]perylene, Indeno[1,2,3-cd]pyrene, Benzo[b]chrysene - 300nm/466nm

Rechtliche Hinweise

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyt

Naphthalene
Acenaphthene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Benzo[a]anthracene
Chrysene
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
Dibenzo[a,h]anthracene
Benzo[g,h,i]perylene
Indeno[1,2,3-cd]pyrene
Benzo[b]chrysene (I.S.)
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