G005624

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis^® Express F5

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Ascentis Express F5, 5 cm x 3.0 mm I.D., 2.7 μm particles (53576-U); mobile phase: [A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile; gradient: 0% B for 1.5 min, 0-10% over 0.5 min; to 15% B in 1.5 min, 2 min re-equilibration at 0% B; flow rate: 1.5 mL/min; pressure: 4931 psi (340 bar); column temp.: 35 °C; detector: UV, 210 nm; injection: 5 μL; sample: all vitamins in water, 10 μg/mL for nicotinic acid and folic acid, 12 μg/mL for thiamine hydrochloride and cyanocobalamin, (20 μg/mL for pyridoxine, 30 μg/mL for riboflavin, 40 μg/mL for niacineamide, 100 μg/mL for calcium pantothenate, 120 μg/mL for biotin)
• Eignung: application for HPLC
• Anwendung(en): vitamins, nutraceuticals, and natural products

Beschreibung

Hinweis zur Analyse

A mix of 9 water-soluble B-vitamins was separated on Ascentis Express F5 column

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

Calcium pantothenate, meets USP testing specifications, monograph mol wt. 476.53 (C₁₈H₃₂CaN₂O₁₀)
Nicotinic acid, ≥99.5% (HPLC)
Pyridoxine hydrochloride, ≥98% (HPLC)
Biotin, ≥99.0% (T)
Nicotinamide, ≥98.5% (HPLC)
Folic acid, analytical standard
Thiamine hydrochloride, reagent grade, ≥99% (HPLC)
Riboflavin (B2), analytical standard
Cyanocobalamin (B12), analytical standard
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