G003763
HPLC Analysis of Cortocisteroids in Urine on Discovery® HS F5 after SPE using Discovery® DSC-CN
application for SPE, application for HPLC
About This Item
Methode(n)
HPLC: suitable
solid phase extraction (SPE): suitable
Testparameter
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: human urine spiked with corticosteroids at 0.5 and 1.0 μg/mL and diluted in DI water (1:1, v/v)
SPE well plate: Discovery DSC-CN 96-well SPE, 100 mg/well (575636-U)
condition: 1 mL methanol; 1 mL DI water.
sample addition: 1 mL spiked urine
washing: 1 mL 20% methanol
elution: 1 mL methanol
eluate post-treatment: Evaporate eluate with nitrogen at 30 °C. Reconstitute in 200 μL mobile phase.
column: Discovery HS F5, 5 cm x 4.6 mm I.D., 3 μm particles (567504-U)
mobile phase: [A] methanol; [B] DI water; (40:60, A:B)
flow rate: 1.5 mL/min
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL
Eignung
application for HPLC
application for SPE
Anwendung(en)
clinical
Rechtliche Hinweise
Choose from one of the most recent versions:
Analysenzertifikate (COA)
Sorry, we don't have COAs for this product available online at this time.
If you need assistance, please contact Kundensupport
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.