813066

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, m-aminophenyl boronate phase

• Synonym(e): TSKgel^® Boronat-5PW HPLC-Säule
• UNSPSC-Code: 41115700

Eigenschaften

• product name: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
• Materialien: PEEK
• Produktlinie: TSKgel^®
• chemische Klasse(n) des Analyten: proteins
• Hersteller/Markenname: Tosoh Boronate-5PW, 13066
• Parameter: ≤20% organic solvent; ≤3 M salt concentration; 1.2 mL/min flow rate; 30 bar max. pressure; ≥ 0 °C temperature
• Methode(n): HPLC: suitable
• L × ID: 7.5 cm × 7.5 mm
• Matrix: polymer particle platform
• Aktive Matrixgruppe: m-aminophenyl boronate phase
• Partikelgröße: 10 μm
• Porengröße: 1000 Å
• Betriebs-pH-Wert: 2-9
• Kapazität: 40 μmol/mL adsorption capacity (sorbitol)
• Kompatibilität: mode of use affinity HPLC
• Trenntechnik: affinity
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

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Rechtliche Hinweise

TSKgel is a registered trademark of Tosoh Corporation

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable