808645

TSKgel^® Chelate-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, iminodiacetic acid phase

• Synonym(e): TSKgel^® Chelat-5PW HPLC-Säule
• UNSPSC-Code: 41115700
• eCl@ss: 32110501

Eigenschaften

• product name: TSKgel^® Chelate-5PW HPLC Column, phase iminodiacetic acid, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
• Materialien: stainless steel
• Produktlinie: TSKgel^®
• Hersteller/Markenname: Tosoh Chelate-5PW, 08645
• Kennzeichnungsgrad: 20 μmol/mL resin ligand density
• Parameter: ≤20% organic solvent; ≤3 M salt concentration; ≤45 °C temperature; 1.2 mL/min flow rate; 10 bar max. pressure
• Methode(n): HPLC: suitable
• L × ID: 7.5 cm × 7.5 mm
• Matrix: polymer particle platform
• Aktive Matrixgruppe: iminodiacetic acid phase
• Partikelgröße: 10 μm
• Porengröße: 1000 Å
• Betriebs-pH-Wert: 2-12
• Kompatibilität: technique used for affinity HPLC
• Trenntechnik: affinity

Beschreibung

Allgemeine Beschreibung

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

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Rechtliche Hinweise

TSKgel is a registered trademark of Tosoh Corporation