796-0477

HPLC Analysis of Cytochrome c Species Variants on Discovery^® BIO PolyMA-SCX (Ion Exchange)

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U); mobile phase: [A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear); flow rate: 0.5 mL/min; column temp.: 25 °C; detector: UV, 280 nm; injection: 10 μg each variant
• Eignung: application for HPLC
• Anwendung(en): life science and biopharma

Beschreibung

Hinweis zur Analyse

This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany