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Merck

53023AST

Supelco

Astec® CLC-D Chiral (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(e):

Chiral Separation CLC-D Column

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About This Item

UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Astec® CLC-D Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Materialien

stainless steel column

Agentur

suitable for USP L32

Produktlinie

Astec®

Verpackung

pkg of 1 ea

Hersteller/Markenname

Astec®

Parameter

0-50 °C temperature
172 bar pressure (2500 psi)

Methode(n)

HPLC: suitable

L × ID

15 cm × 4.6 mm

Matrix

fully porous particle

Aktive Matrixgruppe

chiral bidentate ligand (D-form), requires 5 mM CuSO4 phase

Partikelgröße

5 μm

Porengröße

100 Å

Betriebs-pH-Wert

3.5-7

Trenntechnik

chiral

Allgemeine Beschreibung

CLC columns use the copper ligand concept described by Davankov to effect enantiomer separation (1). The method uses a small, chiral bidentate ligand attached to the silica surface and a copper sulphate-containing mobile phase. The copper ions coordinate with the chiral selector on the stationary phase and carboxylic acid functional groups on the analytes to form transient diastereomeric complexes in solution. The technique also has the advantage of giving small acids with no UV chromophore a strong 254 nm signal. CLC columns are ideal for analysis of hydroxy acids, like lactic, malic, tartaric and mandelic acids, amino acids, other amines and bi-functional racemates, like amino alcohols. Proline and aspartic acid are particularly suited for low-level detection on the CLC column since the copper complex is detected at 254 nm UV. Both can be resolved on the CLC-D or CLC-L in 5 mM CuSO4 with the usual reversal of elution order from the CLC-D to CLC-L. In theory, any analyte that can complete the coordination with the copper ion can be resolved. Two versions of the column provide elution order reversal. On the CLC-D column, the L enantiomer generally elutes before D, with the exception of tartaric acid. The reverse is true on the CLC-L column where D elutes before L.

Features:
  • Separates α-hydroxy carboxylic acids, amino acids and other α-bifunctional compounds
  • High selectivity with simple mobile phases
  • Copper complex gives strong UV 254 nm signal
  • Simple reversal of elution order, CLC-L vs. CLC-D
  • Excellent reproducibility

(1) Davankov, V. A.; Rogozhin, S. V. Ligand chromatography as a novel method for the investigation of mixed complexes: Stereoselective effects in a-amino acid copper(II) complexes. J. Chrom. A. 1971, 60, 284-312.

Rechtliche Hinweise

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

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Die Dokumentenbibliothek aufrufen

Enantiomeric impurities in chiral catalysts, auxiliaries, synthons and resolving agents. Part 2
Armstrong, Daniel W., et al.
Tetrahedron Asymmetry, 10 (1), 37-60 (1999)
Badal C Saha et al.
Biotechnology and bioengineering, 82(7), 864-871 (2003-04-18)
Lactobacillus intermedius B-3693 was selected as a good producer of mannitol from fructose after screening 72 bacterial strains. The bacterium produced mannitol, lactic acid, and acetic acid from fructose in pH-controlled batch fermentation. Typical yields of mannitol, lactic acid, and

Verwandter Inhalt

Astec CLC Copper Ligand Exchange Chiral HPLC Columns. The Astec CLC phases are based on coupling an enantiomeric form of an amine to a proprietary Astec derivative to create an appropriate distance for copper coupling.

Chromatograms

application for HPLCapplication for HPLC

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