Y4770

Anti-YAP1 (C-terminal) in Kaninchen hergestellte Antikörper

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1 65 kDa
• MDL-Nummer: MFCD04118707
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~65 kDa
• Speziesreaktivität: human
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~1 mg/mL
• Methode(n): western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and c-Abl
• UniProt-Hinterlegungsnummer: P46937
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... YAP1(10413) ; mouse ... Yap1(22601) ; rat ... Yap1(363014)

Beschreibung

Allgemeine Beschreibung

YAP1 (Yes-associated protein 1, YAP, YAP2, YAP65) is a modular adaptor protein with multiple protein interaction domains, that is based on its interaction with Src-family tyrosine kinase c-Yes. It has a SH3-binding motif, proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 also contains a 14-3-3 interacting motif.

Spezifität

Anti-YAP1 (C-terminal) antibody is specific for human YAP1. The product is expected to react with mouse, bovine, and dog YAP1. In immunoblotting, detection of the YAP1 band is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 442-454 located at the C-terminus of human YAP1. The sequence is identical in mouse, bovine, and dog YAP1.

Anwendung

Anti-YAP1 (C-terminal) antibody is suitable for use in western blot (.5-1 μg/mL) using HEK-293T cells co-transfected with human YAP1 and c-Abl.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

Biochem./physiol. Wirkung

YAP1 (Yes-associated protein 1) induces p73-dependent apoptosis through the specific and selective co-activation of p53AIP1, an apoptotic p73 target gene. It stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Upon phosphorylation by Akt at Ser¹²⁷, 14-3-3 is recruited and stimulates YAP1 localization to the cytoplasm, resulting in loss of co-activator function in the nucleus. Inhibition of Akt potentiates the nuclear re-localization of YAP1 to promote apoptosis by p73.

YAP1 is a WW domain containing protein that functions as a transcriptional co-activator . It may also regulate p73 gene function in response to DNA damage. YAP1 is known to associate with a wide range of transcription factors . Alterations in YAP1 have been associated with tumor development .

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)