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Key Documents

Y1375

Millipore

YPD-Bouillon

suitable for microbiology, NutriSelect® Basic

Synonym(e):

Yeast Peptone Dextrose Broth

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About This Item

UNSPSC-Code:
41171607
NACRES:
NA.85

Sterilität

non-sterile

Qualitätsniveau

200

Form

powder

Zusammensetzung

Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

Verpackung

poly bottle of 1 kg
poly bottle of 250 g

Hersteller/Markenname

NutriSelect® Basic

Methode(n)

microbiological culture: suitable

Farbe

beige

pH-Wert

6.3-6.7

Anwendung(en)

environmental
food and beverages
microbiology

Eignung

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Verwandte Kategorien

Allgemeine Beschreibung

YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.

Anwendung

YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.

Menge

50 g/L

Angaben zur Herstellung

1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Fußnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Hefeextrakt for use in microbial growth medium

Sigma-Aldrich

Y1625

Hefeextrakt

Christina Bergonzo et al.
Proteins, 88(9), 1189-1196 (2020-03-18)
ClpS2 is a small protein under development as a probe for selectively recognizing N-terminal amino acids of N-degron peptide fragments. To understand the structural basis of ClpS2 specificity for an N-terminal amino acid, all atom molecular dynamics (MD) simulations were
Runhui Liu et al.
Journal of the American Chemical Society, 136(11), 4333-4342 (2014-03-13)
Fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. Previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen Candida albicans
Zuochen Wang et al.
Nature communications, 11(1), 2670-2670 (2020-05-31)
The assembly of active and self-propelled particles is an emerging strategy to create dynamic materials otherwise impossible. However, control of the complex particle interactions remains challenging. Here, we show that various dynamic interactions of active patchy particles can be orchestrated
Veronique Archambault-Leger et al.
Biotechnology for biofuels, 5(1), 49-49 (2012-07-21)
The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal cellulase, and fermentation
Fatemeh Mohammadipanah et al.
Pharmaceutical biology, 56(1), 51-59 (2017-12-26)
Actinobacteria are a precious source of novel bioactive metabolites with potential pharmaceutical applications. Representatives of 11 genera of rare Actinobacteria were selected for the evaluation of antioxidant activity. Fermentation broths of the Actinobacteria were extracted and dosage of 10 to
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Artikel

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protokolle

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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