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V2260

Sigma-Aldrich

Monoklonaler Anti-V5-Antikörper

clone V5-10, purified immunoglobulin, lyophilized powder

Synonym(e):

Monoclonal Anti-V5

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About This Item

MDL-Nummer:
MFCD03457579
UNSPSC-Code:
12352203
NACRES:
NA.56

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

peroxidase conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

V5-10, monoclonal

Form

lyophilized powder

Verpackung

vial of 0.5 mL

Konzentration

5-11 mg/mL

Methode(n)

western blot: 1:4,000-1:8,000

Lagertemp.

2-8°C

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Allgemeine Beschreibung

The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.

Spezifität

Erkennt V5-Tag (GKPIPNPLLGLDST) Fusionsproteine

Anwendung

Suitable for immunoblotting.

Physikalische Form

Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT

Angaben zur Herstellung

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstitute with 0.5 mL of distilled water.

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Panayotis C Theodoropoulos et al.
Nature chemical biology, 12(4), 218-225 (2016-02-02)
A hallmark of targeted cancer therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic at low nanomolar
Steven F Baker et al.
Cell reports, 24(10), 2581-2588 (2018-09-06)
Adaptation of viruses to their hosts can result in specialization and a restricted host range. Species-specific polymorphisms in the influenza virus polymerase restrict its host range during transmission from birds to mammals. ANP32A was recently identified as a cellular co-factor
Vy Tran et al.
Nature microbiology, 5(12), 1490-1503 (2020-08-26)
Cells infected by influenza virus mount a large-scale antiviral response and most cells ultimately initiate cell-death pathways in an attempt to suppress viral replication. We performed a CRISPR-Cas9-knockout selection designed to identify host factors required for replication after viral entry.
Adriana López-Barradas et al.
American journal of physiology. Cell physiology, 311(5), C720-C734 (2016-11-03)
SMCTs move several important fuel molecules that are involved in lipid, carbohydrate, and amino acid metabolism, but their regulation has been poorly studied. Insulin controls the translocation of several solutes that are involved in energetic cellular metabolism, including glucose. We
Moonsup Lee et al.
The Journal of cell biology, 218(8), 2659-2676 (2019-07-05)
Cilia are critical for proper embryonic development and maintaining homeostasis. Although extensively studied, there are still significant gaps regarding the proteins involved in regulating ciliogenesis. Using the Xenopus laevis embryo, we show that Dishevelled (Dvl), a key Wnt signaling scaffold
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