Direkt zum Inhalt
Merck

T9822

Sigma-Aldrich

Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal

clone B3, purified from hybridoma cell culture

Synonym(e):

Anti-K-ALPHA-1

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

B3, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~50 kDa

Speziesreaktivität

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

Methode(n)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

Isotyp

IgM

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).
Tubulin is a cellular colchicine-binding protein.

Immunogen

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Biochem./physiol. Wirkung

Microtubule plays an important role in DNA segregation.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sie haben nicht das passende Produkt gefunden?  

Probieren Sie unser Produkt-Auswahlhilfe. aus.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Parathyroid hormone-related peptide represses chondrocyte hypertrophy through a protein phosphatase 2A/histone deacetylase 4/MEF2 pathway
Kozhemyakina E, et al.
Molecular and Cellular Biology, 29(21), 5751-5762 (2009)
A Mutation in CCDC50, a Gene Encoding an Effector of Epidermal Growth Factor-Mediated Cell Signaling, Causes Progressive Hearing Loss
Modamio-HoybjorS, et al.
American Journal of Human Genetics, 80(6), 1076-1089 (2007)
Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics
Kline-Smith SL and Walczak CE
Molecular Cell, 15(3), 317-327 (2004)
SDCCAG8 interacts with RAB effector proteins RABEP2 and ERC1 and is required for hedgehog signaling
Airik R, et al.
PLoS ONE, 11(5), e0156081-e0156081 (2016)
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain
Ravelli RBG, et al.
Nature, 428(6979), 198-198 (2004)

Artikel

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.