T9378

Trypsin inhibitor

crude powder

• Synonym(e): Ovomucoid
• CAS-Nummer: 9035-81-8
• EG-Nummer: 232-906-9
• MDL-Nummer: MFCD00082102
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Trypsin-Inhibitor aus Phaseolus limensis (lima bean), Type II-L, crude powder
• Biologische Quelle: Phaseolus limensis (lima bean)
• Typ: Type II-L
• Form: crude powder
• Mol-Gew.: 9 kDa
• Löslichkeit: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem./physiol. Wirkung

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Physikalische Eigenschaften

Das Monomer hat ein Molekulargewicht von ca. 9′000, geht aber eine konzentrations- und pH-abhängige Dimerisation ein.

Einheitendefinition

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Angaben zur Herstellung

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Hinweis zur Analyse

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Sonstige Hinweise

View more information on Trypsin Inhibitor.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H317,H334
• P-Sätze: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Gefahreneinstufungen: Resp. Sens. 1 - Skin Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)