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T6778

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Goat Anti-Rabbit IgG (whole molecule)–Tetramethylrhodamine isothiocyanate

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About This Item

MDL-Nummer:
MFCD00162790
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Qualitätsniveau

200

Konjugat

TRITC conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Enthält

≥15 mM sodium azide as preservative

Lagerbedingungen

protect from light

Methode(n)

direct immunofluorescence: 1:400
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Verwandte Kategorien

Allgemeine Beschreibung

Binds all rabbit Igs.
IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds.

Anwendung

Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry.
Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques.

Biochem./physiol. Wirkung

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Sonstige Hinweise

Antibody adsorbed with human IgG.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Angaben zur Herstellung

Adsorbed to reduce background staining with human samples.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Goeh Jung et al.
The Journal of cell biology, 186(4), 555-570 (2009-08-19)
The contractile vacuole (CV) complex in Dictyostelium is a tubulovesicular osmoregulatory organelle that exhibits extensive motility along the actin-rich cortex, providing a useful model for investigating myosin-dependent membrane transport. Here, we show that the type V myosin myoJ localizes to
Kamin J Johnson et al.
Biology of reproduction, 66(4), 992-1000 (2002-03-22)
In the seminiferous epithelium, morphologically diverse junctions mediate inter-Sertoli and Sertoli-germ cell adhesive contact and likely transmit signals between contacting cells. Defining the molecular composition of testicular cell-cell junctions is an important step in determining their function. Proteins belonging to
5-HT1B and 5-HT1D receptors in the human trigeminal ganglion: co-localization with calcitonin gene-related peptide, substance P and nitric oxide synthase
Hou M, et al.
Brain Research, 909(1-2), 112-120 (2001)
Daniel H Fine et al.
Infection and immunity, 73(4), 1947-1953 (2005-03-24)
Cells of the gram-negative periodontopathogen Actinobacillus actinomycetemcomitans express a surface-exposed, outer membrane autotransporter protein, designated Aae, which has been implicated in epithelial cell binding. We constructed a mutant strain of A. actinomycetemcomitans that contained a transposon insertion in the Aae
Martin Hasselblatt et al.
Cancer research, 69(6), 2219-2223 (2009-03-12)
The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid
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Artikel

Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

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