T5425

Transforming Growth Factor-β3 human

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

• Synonym(e): hTGF-β3, TGF-β3
• MDL-Nummer: MFCD00213054
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Transforming Growth Factor-β3 human, TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE)
• Form: powder
• Wirksamkeit: 0.01-0.09 ng/mL ED₅₀/EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: predicted mol wt ~25 kDa
• Verpackung: pkg of 2 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.01 EU/μg
• UniProt-Hinterlegungsnummer: P10600
• Lagertemp.: −20°C
• Angaben zum Gen: human ... TGFB3(7043)

Beschreibung

Allgemeine Beschreibung

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.

Anwendung

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.

Biochem./physiol. Wirkung

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair.

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Hinweis zur Analyse

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)