T3809

TRI-Reagenz^®

BD, For processing whole blood, plasma, or serum.

• Synonym(e): TRI Reagent^® RNA Isolations Reagens
• MDL-Nummer: MFCD00213058
• UNSPSC-Code: 12352200
• NACRES: NA.52

Eigenschaften

• Qualitätsniveau: 200
• Verwendung: 0.75 mL sufficient for 0.25 mL blood derivatives

Beschreibung

Allgemeine Beschreibung

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.

TRI Reagent^® is a monophasic solution of phenol and guanidinium isothiocyanate.

Anwendung

TRI Reagent^® has been used for the isolation of RNA.

The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.

TRI-Reagenz ist eine verbesserte Version des von Chomczynski entwickelten einstufigen Gesamt-RNA-Isolationsreagenzes. Die auf diesem Reagenz basierende RNA-Isolierungsmethode ist für RNA-Anwendungen weit verbreitet und bewährt. Es eignet sich besonders für die schnelle, kostengünstige und effiziente Isolation von Gesamt-RNA oder die gleichzeitige Isolierung von RNA, DNA und Proteinen aus Proben menschlichen, tierischen, pflanzlichen, Hefe-, bakteriellen und viralen Ursprungs.

Leistungsmerkmale und Vorteile

• Leicht skalierbare RNA-Isolation
• Vielseitig einsetzbar mit: Menschen, Pflanzen, Hefe, Bakterien oder Viren
• Bessere Erträge als traditionelle Guanidinthiocyanat/Cäsiumchlorid-Methoden

Rechtliche Hinweise

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Sicherheitshinweise

• Piktogramme: GHS06,GHS08,GHS05,GHS09
• Signalwort: Danger
• H-Sätze: H301 + H311 + H331,H314,H341,H373,H411
• P-Sätze: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Zielorgane: Nervous system,Kidney,Liver,Skin
• Zusätzliche Gefahrenhinweise: EUH032
• Lagerklassenschlüssel: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): 174.2 °F - closed cup
• Flammpunkt (°C): 79 °C - closed cup