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Merck

SRP6274

Sigma-Aldrich

Furin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(e):

FUR, FURIN, PACE, PCSK3, SPC1

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About This Item

UNSPSC-Code:
12352204
NACRES:
NA.32

Biologische Quelle

human

Rekombinant

expressed in HEK 293 cells

Markierung

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

Form

lyophilized powder

Mol-Gew.

calculated mol wt 66.5 kDa
observed mol wt 55-66 kDa (DTT-reduced. Asp 108 is the predicted N-terminus .)

Verpackung

pkg of 10 μg

Verunreinigungen

<1 EU/μg endotoxin (LAL test)

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... PACE(5045)

Allgemeine Beschreibung

Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg′). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.

Physikalische Form

Lyophilized from 0.22 μm filtered solution in MES, Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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