SRP5285

NLK, active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): DKFZp761G1211, FLJ21033
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Produktlinie: PRECISIO^®
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: 1.9-2.9 nmol/min·mg
• Mol-Gew.: ~84 kDa
• NCBI-Hinterlegungsnummer: NM_016231
• Anwendung(en): cell analysis
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... NLK(51701)

Beschreibung

Allgemeine Beschreibung

NLK (also known as nemo-like kinase) is a member of the serine/threonine protein kinase that is encoded by the NLK gene. NLK plays a role in cell fate determination and is required for the differentiation of bone marrow stromal cells. NLK also suppresses a wide range of transcription factors such as CREB by phosphorylation of the co-activator CREBBP. NLK expression increases the number of apoptotic cells but does not induce obvious cell cycle arrest. NLK suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex.

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Angaben zur Herstellung

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable