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SRP5086

Sigma-Aldrich

SLK, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(e):

KIAA0204, LOSK, MGC133067, STK2, bA16H23.1, se20-9

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

Rekombinant

expressed in baculovirus infected Sf9 cells

Produktlinie

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

Form

buffered aqueous glycerol solution

Spezifische Aktivität

3.9-5.3 nmol/min·mg

Mol-Gew.

~180 kDa

NCBI-Hinterlegungsnummer

AB002804

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... SLK(9748)

Allgemeine Beschreibung

SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex. Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity. CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.

Physikalische Form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Angaben zur Herstellung

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Analysenzertifikate (COA)

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LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome.
A V Burakov et al.
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 403, 317-319 (2005-12-20)
Ziad Chaar et al.
The Journal of biological chemistry, 281(38), 28193-28199 (2006-07-14)
We have previously shown that the Ste20-like kinase SLK is a microtubule-associated protein inducing actin stress fiber disassembly. Here, we show that v-Src expression can down-regulate SLK activity. This down-regulation is independent of focal adhesion kinase but requires v-Src kinase

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