SRP3317

ANGPTL-3 human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): ANG-5, ANGPT5
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in CHO cells
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 62.0 kDa
• Verpackung: pkg of 50 μg
• Verunreinigungen: endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: Q9Y5C1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... ANGPTL3(27329)

Beschreibung

Allgemeine Beschreibung

ANGPTL-3 (Angiopoietin like protein 3) is a member of the angiopoietin family of structurally related proteins, characterized by a coiled N-terminal domain and a C-terminal fibrinogen like domain. It is mainly present in the hepatocytes and is also secreted into circulation. The gene is mapped to human chromosome 1p31.
Recombinant human ANGPTL-3 is a glycoprotein that migrates by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis at an apparent molecular weight of 62kDa, and contains 452 amino acid residues including a C-terminal His tag.

Biochem./physiol. Wirkung

ANGPTL-3 (Angiopoietin like protein 3) can exert activities related to both angiogenesis and lipid metabolism. ANGPTL-3 inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), which has the effect of increasing plasma levels of triglycerides and HDL (high-density lipoprotein)-associated cholesterol. The fibrinogen like portion of the ANGPTL-3 protein can bind α-5/β-3 integrins leading to endothelial cell adhesion and migration. Mutations in this gene can result in familial combined hypobetalipoproteinemia.

Physikalische Form

Lyophilized from 10mM Sodium Phosphate, pH 7.5.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable