SRP3292

R-SPONDIN-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(e): Rspo1
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in CHO cells
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: 1.0-3.0 μg/mL ED₅₀
• Mol-Gew.: 26.0-40.0 kDa
• Verpackung: pkg of 20 μg
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white
• UniProt-Hinterlegungsnummer: Q2MKA7
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... RSPO1(284654)

Beschreibung

Allgemeine Beschreibung

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the (Rspo) family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Recombinant human R-Spondin-1 is a 26.7kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0kDa by SDS PAGE analysis under reducing conditions.

Biochem./physiol. Wirkung

Rspo (R-Spondin) can interact with the Frizzled/LRP6 (LDL receptor related protein 6) receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Disruption of Rspo-1 gene causes recessive syndrome with XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Rspo-1 is also associated with radioresistance of tumor cells.

Physikalische Form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable