SRP2115

RNA Polymerase II, p33 subunit, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(e): RPB3, RPB31, hRPB33, hsRPB3
• UNSPSC-Code: 12352202
• NACRES: NA.26

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• Form: frozen liquid
• Mol-Gew.: ~59.1 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: 150 μg/mL
• Farbe: clear colorless
• NCBI-Hinterlegungsnummer: NM_032940
• UniProt-Hinterlegungsnummer: P19387
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... POLR2C(5432)

Beschreibung

Biochem./physiol. Wirkung

In human, RPB3 is encoded by the POLR2C gene. This gene encodes the third largest subunit of RNA polymerase II. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. In the pol II complex, RPB3 interacts with RPB5 and the RPB3-RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11. Besides, RPB3 also interacts with RPB. RPB3 is involved in tissue-specific transcription and muscle differentiation via interaction with the myogenic factor Myogenin. RPB3 also interact with IGFBP3.

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable