SRP2082

WT-1 (+KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

• UNSPSC-Code: 12352200
• NACRES: NA.26

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in insect cells
• Assay: ≥60% (SDS-PAGE)
• Form: frozen liquid
• Verpackung: pkg of 5 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: 150 μg/mL
• Farbe: clear colorless
• NCBI-Hinterlegungsnummer: NM_024425
• UniProt-Hinterlegungsnummer: P19544
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... WT1(7490)

Beschreibung

Biochem./physiol. Wirkung

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable