SRP0735

LIMK2, Active

PRECISIO^® Kinase, recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: 2.5-3.5 nmol/min·mg (radiometric)
• Mol-Gew.: ~96 kDa
• Konzentration: 0.05 μg/μL
• Hinterlegungsnummer: CR456513
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... LIMK2(3985)

Beschreibung

Anwendung

Useful for kinase assay and Western blot.

Biochem./physiol. Wirkung

Recombinant full-length human LIMK2 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. LIMK2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain ⁽¹⁾. LIM domains are highly conserved cysteine-rich structures that can mediate protein-protein interaction. LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and then LIMK2 in turn phosphorylates cofilin, inhibiting its actin-depolymerizing activity which contributes to Rho-induced reorganization of the actin cytoskeleton ⁽²⁾. LIMK2 is highly expressed in liver, colon, stomach, spleen, brain, kidney, and placenta.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable