SRP0258

PDE1B Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(e): Calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase, Cam-PDE 63 kDa, PDES1B, Phosphodiesterase 1B
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Spezifische Aktivität: ≥2000 units/μg
• Mol-Gew.: 87 kDa
• Verpackung: pkg of 10 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_000924
• UniProt-Hinterlegungsnummer: Q01064
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PDE1B(5153)

Beschreibung

Allgemeine Beschreibung

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.