SRP0136

G9a human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(e): EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 74.6 kDa
• Verpackung: pkg of 20 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_006709
• UniProt-Hinterlegungsnummer: Q96KQ7
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... EHMT2(10919)

Beschreibung

Allgemeine Beschreibung

Human G9a, euchromatic histone-lysine N-methyltransferase 2, GenBank Accession No. NM_006709, amino acids 785-1210, with N-terminal GST tag, MW = 74.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.