SHC015V

MISSION^® pLKO.1-puro-UbC-TagFP635^™ Positive Control Transduction Particles

Contains a gene encoding TagFP635, under the control of the UbC promoter

• Synonym(e): MISSION^®, MISSION^® Control Transduction Particles
• UNSPSC-Code: 12352200

Eigenschaften

• Produktlinie: MISSION^®
• Methode(n): capture ELISA: 10⁶ TU/mL using p24
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Anwendung

To see more application data, protocols, vector maps visit sigma.com/shrna.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TagFP635 Control Transduction Particles are produced from the sequence-verified 9226 base pair lentivirus plasmid, pLKO.1-puro-UbC-TagFP635 (SHC015).

This construct aids in interpretation of experimental design and results by providing a far-red fluorescent protein marker for monitoring success in transduction of your cells of interest.

Rechtliche Hinweise

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TagFP635 is a trademark of Evrogen Co.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable